Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
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چکیده
منابع مشابه
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-(3)H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide gels. The distribution of total or newly synthesized proteins from the single neurons was determined by staining or slicing and liquid scintill...
متن کاملSynaptogenesis by single identified neurons in vitro: contribution of rapidly transported and newly synthesized proteins.
The object of these studies is to define the molecular events that occur during synaptogenesis. Our approach is to use single identified Aplysia neurons grown in culture under conditions where chemical synapses are formed. In this report we studied synapses established by R2, a giant cholinergic neuron, onto neurons R15 and L11, and a group of left upper quadrant (LUQ) cells. The detailed elect...
متن کاملAxonal Transport of Newly Synthesized Glycoproteins in a Single Identified Neuron of Aplysia Californica
Increasing amounts of glycoprotein synthesized from L-[ 3H]fucose injected into the cell body of R2, an identified Aplysia neuron, were found in the right pleuroabdominal connective . Autoradiography revealed that the glycoproteins were localized in the axon of R2. Glycoproteins appearing in the axon presumably were synthesized in the cell body, since no significant incorporation was observed w...
متن کاملAxonal Transport of Newly Synthesized Glycoproteins in a Single Identified Neuron of Aplysia Californica
Increasing amounts of glycoprotein synthesized from L-[(3)H]fucose injected into the cell body of R2, an identified Aplysia neuron, were found in the right pleuro-abdominal connective. Autoradiography revealed that the glycoproteins were localized in the axon of R2. Glycoproteins appearing in the axon presumably were synthesized in the cell body, since no significant incorporation was observed ...
متن کاملIsolated identified Aplysia neurons in cell culture.
Methods have been developed for primary culture of large identified Aplysia neurons. Aplysia ganglia were treated with neutral protease to soften the connective tissue sheath. Individual neurons were isolated either by manipulation with tungsten needles or by tying off their axons with fine nylon filament and were immobilized in a chick plasma clot or a solution of methylcellulose. Somata up to...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1971
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.57.1.26